evaluation of the chlroplast dna among vicia faba l. germplasm using restriction- site analysis

a restriction-site analysis of chloroplast dna (cpdna) was carried out to evaluate the levelof diversity in vicia faba l. germplasm selected from different geographical regions using 11 restrictionendonucleases. we analyzed 214 restriction sites in 18 accessions of vicia faba. all of the accessionshad identical cpdnas, pointing out the ancestral character of all the accessions are of only one genepool, and all of them must have evolved through the same maternal lineage. molecular size of thechloroplast dnas obtained was 123.25 kb, indicating that it had lost one of the inverted repeats. due tolack of cpdna diversity, it is concluded that the broad bean has passed through a genetic bottleneckduring domestication and lost most of its cytoplasmic variability. the present study favors themonophyletic origin of its cytoplasm, and accordingly, of vicia faba.